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aavs1 cmv ostir1 f74g  (Addgene inc)


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    Addgene inc aavs1 cmv ostir1 f74g
    Aavs1 Cmv Ostir1 F74g, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ostir1+f74g/pMK381+(AAVS1+CMV-OsTIR1F74G)+(Plasmid+%23140536)/bio_rxiv__64898__2025__12__17__694947-33-7-13
    Average 93 stars, based on 15 article reviews
    aavs1 cmv ostir1 f74g - by Bioz Stars, 2026-10
    93/100 stars

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    Article Title: Revealing Acute Consequences of Rapid Protein Elimination at Individual Synapses using Auxin-Inducible Degron 2 Technology
    Article Snippet: All gene synthesis and cloning were done by Genscript (Piscataway NJ, USA). .. Lentiviral vectors for expressing OsTIR1(F74G) in neurons in culture were created as follows: The pAAV-hSyn-OsTIR1(F74G) plasmid described in was obtained from Addgene (Addgene #140730). ..

    Plasmid Preparation:

    Article Title: Revealing Acute Consequences of Rapid Protein Elimination at Individual Synapses using Auxin-Inducible Degron 2 Technology
    Article Snippet: All gene synthesis and cloning were done by Genscript (Piscataway NJ, USA). .. Lentiviral vectors for expressing OsTIR1(F74G) in neurons in culture were created as follows: The pAAV-hSyn-OsTIR1(F74G) plasmid described in was obtained from Addgene (Addgene #140730). ..



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    Aavs1 Cmv Ostir1 F74g, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ostir1+f74g/pMK381+(AAVS1+CMV-OsTIR1F74G)+(Plasmid+%23140536)/bio_rxiv__64898__2025__12__17__694947-33-7-13
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    pay15 ostir1 f74g maid egfp nls  (Addgene inc)
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    A CUT&RUN analysis to assess c-Rel genomic binding. Fluorescence microscopy imaging of the <t>Rel-pAY15</t> stably transfected A2929 cell line. c-Rel is tagged with GFP. Lepto-B: Leptomycin-B. Heatmap showing the genomic regions differentially bound by c-Rel. The peak numbers for the genomic locations where c-Rel is bound are given in a pie chart. The CUT&RUN and RNAseq tracks display the expression levels of the Fn1 and Itgb3 genes in mock-transfected (pAY15), c-Rel-overexpressing (Rel-pAY15), and degron-induced c-Rel-overexpressing A2929 (Rel-pAY15 + 5phIAA) cell lines. B Normalized RT‒qPCR analysis for the selected targets. Stably transfected cells are cultured under adherent (2D) or nonadherent conditions (3D) (A2929 c-Rel expression factor, Itgb3 p= 0.0006 , ItgaV p=0.0001 , Itga5 p=0.0069 , total Fn1 p=0.0014 , EDA+ Fn1 p<0.0001 , EDB+ Fn1 p=0.0004; for A2770 c-Rel expression factor Itgb3 p=0.0011 , ItgaV p=0.0032 , Itga5 p=0.0014 , total Fn1 p=0.0027 , EDA+ Fn1 p<0.0001 , EDB+ Fn1 p<0.0001). C Overrepresentation analysis performed by Enrichr for Rel-pAY15 cells. The analysis is performed only with the genes that were differentially expressed in Rel-pAY15 cells compared with those in pAY15-only cells or in Rel-pAY15-induced degron cells. From each set, only the top 5 hits are selected on the basis of their combined scores. D Correlation of the REL with FN1 and ITGB3 mRNA expression in primary tumor samples from the TCGA_PAAD cohort. The graphs are adapted with data from the UCSC-Xena TCGA PAAD dataset. The Pearson r correlations are displayed in the figure for each target. E Patient TMA H-score correlation plots for c-Rel versus ITGB3 or FN1. F) Kaplan–Meier survival curves for patients in the TCGA dataset, divided by the median expression level, on the basis of either FN1 or ITGB3 expression. OS: overall survival, n= 178; DSS: disease-specific survival, n=172; DFI: disease-free interval, n=69; PFI: progression-free interval, n=178. The graphs are adapted with data from the UCSC-Xena TCGA PAAD dataset. G Patient TMA overall survival curves based on ITGB3 and FN1 H-scores. Statistical analysis is performed with the log-rank (Mantel‒Cox) test. Unless otherwise indicated, for all analyses, two-way ANOVA is used. Šídák's multiple comparison tests between genotypes are shown in the figures
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    A CUT&RUN analysis to assess c-Rel genomic binding. Fluorescence microscopy imaging of the <t>Rel-pAY15</t> stably transfected A2929 cell line. c-Rel is tagged with GFP. Lepto-B: Leptomycin-B. Heatmap showing the genomic regions differentially bound by c-Rel. The peak numbers for the genomic locations where c-Rel is bound are given in a pie chart. The CUT&RUN and RNAseq tracks display the expression levels of the Fn1 and Itgb3 genes in mock-transfected (pAY15), c-Rel-overexpressing (Rel-pAY15), and degron-induced c-Rel-overexpressing A2929 (Rel-pAY15 + 5phIAA) cell lines. B Normalized RT‒qPCR analysis for the selected targets. Stably transfected cells are cultured under adherent (2D) or nonadherent conditions (3D) (A2929 c-Rel expression factor, Itgb3 p= 0.0006 , ItgaV p=0.0001 , Itga5 p=0.0069 , total Fn1 p=0.0014 , EDA+ Fn1 p<0.0001 , EDB+ Fn1 p=0.0004; for A2770 c-Rel expression factor Itgb3 p=0.0011 , ItgaV p=0.0032 , Itga5 p=0.0014 , total Fn1 p=0.0027 , EDA+ Fn1 p<0.0001 , EDB+ Fn1 p<0.0001). C Overrepresentation analysis performed by Enrichr for Rel-pAY15 cells. The analysis is performed only with the genes that were differentially expressed in Rel-pAY15 cells compared with those in pAY15-only cells or in Rel-pAY15-induced degron cells. From each set, only the top 5 hits are selected on the basis of their combined scores. D Correlation of the REL with FN1 and ITGB3 mRNA expression in primary tumor samples from the TCGA_PAAD cohort. The graphs are adapted with data from the UCSC-Xena TCGA PAAD dataset. The Pearson r correlations are displayed in the figure for each target. E Patient TMA H-score correlation plots for c-Rel versus ITGB3 or FN1. F) Kaplan–Meier survival curves for patients in the TCGA dataset, divided by the median expression level, on the basis of either FN1 or ITGB3 expression. OS: overall survival, n= 178; DSS: disease-specific survival, n=172; DFI: disease-free interval, n=69; PFI: progression-free interval, n=178. The graphs are adapted with data from the UCSC-Xena TCGA PAAD dataset. G Patient TMA overall survival curves based on ITGB3 and FN1 H-scores. Statistical analysis is performed with the log-rank (Mantel‒Cox) test. Unless otherwise indicated, for all analyses, two-way ANOVA is used. Šídák's multiple comparison tests between genotypes are shown in the figures
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    A CUT&RUN analysis to assess c-Rel genomic binding. Fluorescence microscopy imaging of the <t>Rel-pAY15</t> stably transfected A2929 cell line. c-Rel is tagged with GFP. Lepto-B: Leptomycin-B. Heatmap showing the genomic regions differentially bound by c-Rel. The peak numbers for the genomic locations where c-Rel is bound are given in a pie chart. The CUT&RUN and RNAseq tracks display the expression levels of the Fn1 and Itgb3 genes in mock-transfected (pAY15), c-Rel-overexpressing (Rel-pAY15), and degron-induced c-Rel-overexpressing A2929 (Rel-pAY15 + 5phIAA) cell lines. B Normalized RT‒qPCR analysis for the selected targets. Stably transfected cells are cultured under adherent (2D) or nonadherent conditions (3D) (A2929 c-Rel expression factor, Itgb3 p= 0.0006 , ItgaV p=0.0001 , Itga5 p=0.0069 , total Fn1 p=0.0014 , EDA+ Fn1 p<0.0001 , EDB+ Fn1 p=0.0004; for A2770 c-Rel expression factor Itgb3 p=0.0011 , ItgaV p=0.0032 , Itga5 p=0.0014 , total Fn1 p=0.0027 , EDA+ Fn1 p<0.0001 , EDB+ Fn1 p<0.0001). C Overrepresentation analysis performed by Enrichr for Rel-pAY15 cells. The analysis is performed only with the genes that were differentially expressed in Rel-pAY15 cells compared with those in pAY15-only cells or in Rel-pAY15-induced degron cells. From each set, only the top 5 hits are selected on the basis of their combined scores. D Correlation of the REL with FN1 and ITGB3 mRNA expression in primary tumor samples from the TCGA_PAAD cohort. The graphs are adapted with data from the UCSC-Xena TCGA PAAD dataset. The Pearson r correlations are displayed in the figure for each target. E Patient TMA H-score correlation plots for c-Rel versus ITGB3 or FN1. F) Kaplan–Meier survival curves for patients in the TCGA dataset, divided by the median expression level, on the basis of either FN1 or ITGB3 expression. OS: overall survival, n= 178; DSS: disease-specific survival, n=172; DFI: disease-free interval, n=69; PFI: progression-free interval, n=178. The graphs are adapted with data from the UCSC-Xena TCGA PAAD dataset. G Patient TMA overall survival curves based on ITGB3 and FN1 H-scores. Statistical analysis is performed with the log-rank (Mantel‒Cox) test. Unless otherwise indicated, for all analyses, two-way ANOVA is used. Šídák's multiple comparison tests between genotypes are shown in the figures
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    A CUT&RUN analysis to assess c-Rel genomic binding. Fluorescence microscopy imaging of the <t>Rel-pAY15</t> stably transfected A2929 cell line. c-Rel is tagged with GFP. Lepto-B: Leptomycin-B. Heatmap showing the genomic regions differentially bound by c-Rel. The peak numbers for the genomic locations where c-Rel is bound are given in a pie chart. The CUT&RUN and RNAseq tracks display the expression levels of the Fn1 and Itgb3 genes in mock-transfected (pAY15), c-Rel-overexpressing (Rel-pAY15), and degron-induced c-Rel-overexpressing A2929 (Rel-pAY15 + 5phIAA) cell lines. B Normalized RT‒qPCR analysis for the selected targets. Stably transfected cells are cultured under adherent (2D) or nonadherent conditions (3D) (A2929 c-Rel expression factor, Itgb3 p= 0.0006 , ItgaV p=0.0001 , Itga5 p=0.0069 , total Fn1 p=0.0014 , EDA+ Fn1 p<0.0001 , EDB+ Fn1 p=0.0004; for A2770 c-Rel expression factor Itgb3 p=0.0011 , ItgaV p=0.0032 , Itga5 p=0.0014 , total Fn1 p=0.0027 , EDA+ Fn1 p<0.0001 , EDB+ Fn1 p<0.0001). C Overrepresentation analysis performed by Enrichr for Rel-pAY15 cells. The analysis is performed only with the genes that were differentially expressed in Rel-pAY15 cells compared with those in pAY15-only cells or in Rel-pAY15-induced degron cells. From each set, only the top 5 hits are selected on the basis of their combined scores. D Correlation of the REL with FN1 and ITGB3 mRNA expression in primary tumor samples from the TCGA_PAAD cohort. The graphs are adapted with data from the UCSC-Xena TCGA PAAD dataset. The Pearson r correlations are displayed in the figure for each target. E Patient TMA H-score correlation plots for c-Rel versus ITGB3 or FN1. F) Kaplan–Meier survival curves for patients in the TCGA dataset, divided by the median expression level, on the basis of either FN1 or ITGB3 expression. OS: overall survival, n= 178; DSS: disease-specific survival, n=172; DFI: disease-free interval, n=69; PFI: progression-free interval, n=178. The graphs are adapted with data from the UCSC-Xena TCGA PAAD dataset. G Patient TMA overall survival curves based on ITGB3 and FN1 H-scores. Statistical analysis is performed with the log-rank (Mantel‒Cox) test. Unless otherwise indicated, for all analyses, two-way ANOVA is used. Šídák's multiple comparison tests between genotypes are shown in the figures
    Paav Hsyn Ostir1 F74g Plasmid25, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    A CUT&RUN analysis to assess c-Rel genomic binding. Fluorescence microscopy imaging of the <t>Rel-pAY15</t> stably transfected A2929 cell line. c-Rel is tagged with GFP. Lepto-B: Leptomycin-B. Heatmap showing the genomic regions differentially bound by c-Rel. The peak numbers for the genomic locations where c-Rel is bound are given in a pie chart. The CUT&RUN and RNAseq tracks display the expression levels of the Fn1 and Itgb3 genes in mock-transfected (pAY15), c-Rel-overexpressing (Rel-pAY15), and degron-induced c-Rel-overexpressing A2929 (Rel-pAY15 + 5phIAA) cell lines. B Normalized RT‒qPCR analysis for the selected targets. Stably transfected cells are cultured under adherent (2D) or nonadherent conditions (3D) (A2929 c-Rel expression factor, Itgb3 p= 0.0006 , ItgaV p=0.0001 , Itga5 p=0.0069 , total Fn1 p=0.0014 , EDA+ Fn1 p<0.0001 , EDB+ Fn1 p=0.0004; for A2770 c-Rel expression factor Itgb3 p=0.0011 , ItgaV p=0.0032 , Itga5 p=0.0014 , total Fn1 p=0.0027 , EDA+ Fn1 p<0.0001 , EDB+ Fn1 p<0.0001). C Overrepresentation analysis performed by Enrichr for Rel-pAY15 cells. The analysis is performed only with the genes that were differentially expressed in Rel-pAY15 cells compared with those in pAY15-only cells or in Rel-pAY15-induced degron cells. From each set, only the top 5 hits are selected on the basis of their combined scores. D Correlation of the REL with FN1 and ITGB3 mRNA expression in primary tumor samples from the TCGA_PAAD cohort. The graphs are adapted with data from the UCSC-Xena TCGA PAAD dataset. The Pearson r correlations are displayed in the figure for each target. E Patient TMA H-score correlation plots for c-Rel versus ITGB3 or FN1. F) Kaplan–Meier survival curves for patients in the TCGA dataset, divided by the median expression level, on the basis of either FN1 or ITGB3 expression. OS: overall survival, n= 178; DSS: disease-specific survival, n=172; DFI: disease-free interval, n=69; PFI: progression-free interval, n=178. The graphs are adapted with data from the UCSC-Xena TCGA PAAD dataset. G Patient TMA overall survival curves based on ITGB3 and FN1 H-scores. Statistical analysis is performed with the log-rank (Mantel‒Cox) test. Unless otherwise indicated, for all analyses, two-way ANOVA is used. Šídák's multiple comparison tests between genotypes are shown in the figures
    Fromaddgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Addgene inc aav8 hsyn ostir1 f74g p2a maid egfp nes25 addgene
    A CUT&RUN analysis to assess c-Rel genomic binding. Fluorescence microscopy imaging of the <t>Rel-pAY15</t> stably transfected A2929 cell line. c-Rel is tagged with GFP. Lepto-B: Leptomycin-B. Heatmap showing the genomic regions differentially bound by c-Rel. The peak numbers for the genomic locations where c-Rel is bound are given in a pie chart. The CUT&RUN and RNAseq tracks display the expression levels of the Fn1 and Itgb3 genes in mock-transfected (pAY15), c-Rel-overexpressing (Rel-pAY15), and degron-induced c-Rel-overexpressing A2929 (Rel-pAY15 + 5phIAA) cell lines. B Normalized RT‒qPCR analysis for the selected targets. Stably transfected cells are cultured under adherent (2D) or nonadherent conditions (3D) (A2929 c-Rel expression factor, Itgb3 p= 0.0006 , ItgaV p=0.0001 , Itga5 p=0.0069 , total Fn1 p=0.0014 , EDA+ Fn1 p<0.0001 , EDB+ Fn1 p=0.0004; for A2770 c-Rel expression factor Itgb3 p=0.0011 , ItgaV p=0.0032 , Itga5 p=0.0014 , total Fn1 p=0.0027 , EDA+ Fn1 p<0.0001 , EDB+ Fn1 p<0.0001). C Overrepresentation analysis performed by Enrichr for Rel-pAY15 cells. The analysis is performed only with the genes that were differentially expressed in Rel-pAY15 cells compared with those in pAY15-only cells or in Rel-pAY15-induced degron cells. From each set, only the top 5 hits are selected on the basis of their combined scores. D Correlation of the REL with FN1 and ITGB3 mRNA expression in primary tumor samples from the TCGA_PAAD cohort. The graphs are adapted with data from the UCSC-Xena TCGA PAAD dataset. The Pearson r correlations are displayed in the figure for each target. E Patient TMA H-score correlation plots for c-Rel versus ITGB3 or FN1. F) Kaplan–Meier survival curves for patients in the TCGA dataset, divided by the median expression level, on the basis of either FN1 or ITGB3 expression. OS: overall survival, n= 178; DSS: disease-specific survival, n=172; DFI: disease-free interval, n=69; PFI: progression-free interval, n=178. The graphs are adapted with data from the UCSC-Xena TCGA PAAD dataset. G Patient TMA overall survival curves based on ITGB3 and FN1 H-scores. Statistical analysis is performed with the log-rank (Mantel‒Cox) test. Unless otherwise indicated, for all analyses, two-way ANOVA is used. Šídák's multiple comparison tests between genotypes are shown in the figures
    Aav8 Hsyn Ostir1 F74g P2a Maid Egfp Nes25 Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    paav hsyn ostir1 f74g plasmid  (Addgene inc)
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    A CUT&RUN analysis to assess c-Rel genomic binding. Fluorescence microscopy imaging of the <t>Rel-pAY15</t> stably transfected A2929 cell line. c-Rel is tagged with GFP. Lepto-B: Leptomycin-B. Heatmap showing the genomic regions differentially bound by c-Rel. The peak numbers for the genomic locations where c-Rel is bound are given in a pie chart. The CUT&RUN and RNAseq tracks display the expression levels of the Fn1 and Itgb3 genes in mock-transfected (pAY15), c-Rel-overexpressing (Rel-pAY15), and degron-induced c-Rel-overexpressing A2929 (Rel-pAY15 + 5phIAA) cell lines. B Normalized RT‒qPCR analysis for the selected targets. Stably transfected cells are cultured under adherent (2D) or nonadherent conditions (3D) (A2929 c-Rel expression factor, Itgb3 p= 0.0006 , ItgaV p=0.0001 , Itga5 p=0.0069 , total Fn1 p=0.0014 , EDA+ Fn1 p<0.0001 , EDB+ Fn1 p=0.0004; for A2770 c-Rel expression factor Itgb3 p=0.0011 , ItgaV p=0.0032 , Itga5 p=0.0014 , total Fn1 p=0.0027 , EDA+ Fn1 p<0.0001 , EDB+ Fn1 p<0.0001). C Overrepresentation analysis performed by Enrichr for Rel-pAY15 cells. The analysis is performed only with the genes that were differentially expressed in Rel-pAY15 cells compared with those in pAY15-only cells or in Rel-pAY15-induced degron cells. From each set, only the top 5 hits are selected on the basis of their combined scores. D Correlation of the REL with FN1 and ITGB3 mRNA expression in primary tumor samples from the TCGA_PAAD cohort. The graphs are adapted with data from the UCSC-Xena TCGA PAAD dataset. The Pearson r correlations are displayed in the figure for each target. E Patient TMA H-score correlation plots for c-Rel versus ITGB3 or FN1. F) Kaplan–Meier survival curves for patients in the TCGA dataset, divided by the median expression level, on the basis of either FN1 or ITGB3 expression. OS: overall survival, n= 178; DSS: disease-specific survival, n=172; DFI: disease-free interval, n=69; PFI: progression-free interval, n=178. The graphs are adapted with data from the UCSC-Xena TCGA PAAD dataset. G Patient TMA overall survival curves based on ITGB3 and FN1 H-scores. Statistical analysis is performed with the log-rank (Mantel‒Cox) test. Unless otherwise indicated, for all analyses, two-way ANOVA is used. Šídák's multiple comparison tests between genotypes are shown in the figures
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    aav8 hsyn ostir1 f74g p2a maid egfp nes addgene  (Addgene inc)
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    Addgene inc aav8 hsyn ostir1 f74g p2a maid egfp nes addgene
    A CUT&RUN analysis to assess c-Rel genomic binding. Fluorescence microscopy imaging of the <t>Rel-pAY15</t> stably transfected A2929 cell line. c-Rel is tagged with GFP. Lepto-B: Leptomycin-B. Heatmap showing the genomic regions differentially bound by c-Rel. The peak numbers for the genomic locations where c-Rel is bound are given in a pie chart. The CUT&RUN and RNAseq tracks display the expression levels of the Fn1 and Itgb3 genes in mock-transfected (pAY15), c-Rel-overexpressing (Rel-pAY15), and degron-induced c-Rel-overexpressing A2929 (Rel-pAY15 + 5phIAA) cell lines. B Normalized RT‒qPCR analysis for the selected targets. Stably transfected cells are cultured under adherent (2D) or nonadherent conditions (3D) (A2929 c-Rel expression factor, Itgb3 p= 0.0006 , ItgaV p=0.0001 , Itga5 p=0.0069 , total Fn1 p=0.0014 , EDA+ Fn1 p<0.0001 , EDB+ Fn1 p=0.0004; for A2770 c-Rel expression factor Itgb3 p=0.0011 , ItgaV p=0.0032 , Itga5 p=0.0014 , total Fn1 p=0.0027 , EDA+ Fn1 p<0.0001 , EDB+ Fn1 p<0.0001). C Overrepresentation analysis performed by Enrichr for Rel-pAY15 cells. The analysis is performed only with the genes that were differentially expressed in Rel-pAY15 cells compared with those in pAY15-only cells or in Rel-pAY15-induced degron cells. From each set, only the top 5 hits are selected on the basis of their combined scores. D Correlation of the REL with FN1 and ITGB3 mRNA expression in primary tumor samples from the TCGA_PAAD cohort. The graphs are adapted with data from the UCSC-Xena TCGA PAAD dataset. The Pearson r correlations are displayed in the figure for each target. E Patient TMA H-score correlation plots for c-Rel versus ITGB3 or FN1. F) Kaplan–Meier survival curves for patients in the TCGA dataset, divided by the median expression level, on the basis of either FN1 or ITGB3 expression. OS: overall survival, n= 178; DSS: disease-specific survival, n=172; DFI: disease-free interval, n=69; PFI: progression-free interval, n=178. The graphs are adapted with data from the UCSC-Xena TCGA PAAD dataset. G Patient TMA overall survival curves based on ITGB3 and FN1 H-scores. Statistical analysis is performed with the log-rank (Mantel‒Cox) test. Unless otherwise indicated, for all analyses, two-way ANOVA is used. Šídák's multiple comparison tests between genotypes are shown in the figures
    Aav8 Hsyn Ostir1 F74g P2a Maid Egfp Nes Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    A CUT&RUN analysis to assess c-Rel genomic binding. Fluorescence microscopy imaging of the Rel-pAY15 stably transfected A2929 cell line. c-Rel is tagged with GFP. Lepto-B: Leptomycin-B. Heatmap showing the genomic regions differentially bound by c-Rel. The peak numbers for the genomic locations where c-Rel is bound are given in a pie chart. The CUT&RUN and RNAseq tracks display the expression levels of the Fn1 and Itgb3 genes in mock-transfected (pAY15), c-Rel-overexpressing (Rel-pAY15), and degron-induced c-Rel-overexpressing A2929 (Rel-pAY15 + 5phIAA) cell lines. B Normalized RT‒qPCR analysis for the selected targets. Stably transfected cells are cultured under adherent (2D) or nonadherent conditions (3D) (A2929 c-Rel expression factor, Itgb3 p= 0.0006 , ItgaV p=0.0001 , Itga5 p=0.0069 , total Fn1 p=0.0014 , EDA+ Fn1 p<0.0001 , EDB+ Fn1 p=0.0004; for A2770 c-Rel expression factor Itgb3 p=0.0011 , ItgaV p=0.0032 , Itga5 p=0.0014 , total Fn1 p=0.0027 , EDA+ Fn1 p<0.0001 , EDB+ Fn1 p<0.0001). C Overrepresentation analysis performed by Enrichr for Rel-pAY15 cells. The analysis is performed only with the genes that were differentially expressed in Rel-pAY15 cells compared with those in pAY15-only cells or in Rel-pAY15-induced degron cells. From each set, only the top 5 hits are selected on the basis of their combined scores. D Correlation of the REL with FN1 and ITGB3 mRNA expression in primary tumor samples from the TCGA_PAAD cohort. The graphs are adapted with data from the UCSC-Xena TCGA PAAD dataset. The Pearson r correlations are displayed in the figure for each target. E Patient TMA H-score correlation plots for c-Rel versus ITGB3 or FN1. F) Kaplan–Meier survival curves for patients in the TCGA dataset, divided by the median expression level, on the basis of either FN1 or ITGB3 expression. OS: overall survival, n= 178; DSS: disease-specific survival, n=172; DFI: disease-free interval, n=69; PFI: progression-free interval, n=178. The graphs are adapted with data from the UCSC-Xena TCGA PAAD dataset. G Patient TMA overall survival curves based on ITGB3 and FN1 H-scores. Statistical analysis is performed with the log-rank (Mantel‒Cox) test. Unless otherwise indicated, for all analyses, two-way ANOVA is used. Šídák's multiple comparison tests between genotypes are shown in the figures

    Journal: Molecular Cancer

    Article Title: c-Rel drives pancreatic cancer metastasis through fibronectin-integrin signaling-induced isolation stress resistance and EMT

    doi: 10.1186/s12943-025-02486-5

    Figure Lengend Snippet: A CUT&RUN analysis to assess c-Rel genomic binding. Fluorescence microscopy imaging of the Rel-pAY15 stably transfected A2929 cell line. c-Rel is tagged with GFP. Lepto-B: Leptomycin-B. Heatmap showing the genomic regions differentially bound by c-Rel. The peak numbers for the genomic locations where c-Rel is bound are given in a pie chart. The CUT&RUN and RNAseq tracks display the expression levels of the Fn1 and Itgb3 genes in mock-transfected (pAY15), c-Rel-overexpressing (Rel-pAY15), and degron-induced c-Rel-overexpressing A2929 (Rel-pAY15 + 5phIAA) cell lines. B Normalized RT‒qPCR analysis for the selected targets. Stably transfected cells are cultured under adherent (2D) or nonadherent conditions (3D) (A2929 c-Rel expression factor, Itgb3 p= 0.0006 , ItgaV p=0.0001 , Itga5 p=0.0069 , total Fn1 p=0.0014 , EDA+ Fn1 p<0.0001 , EDB+ Fn1 p=0.0004; for A2770 c-Rel expression factor Itgb3 p=0.0011 , ItgaV p=0.0032 , Itga5 p=0.0014 , total Fn1 p=0.0027 , EDA+ Fn1 p<0.0001 , EDB+ Fn1 p<0.0001). C Overrepresentation analysis performed by Enrichr for Rel-pAY15 cells. The analysis is performed only with the genes that were differentially expressed in Rel-pAY15 cells compared with those in pAY15-only cells or in Rel-pAY15-induced degron cells. From each set, only the top 5 hits are selected on the basis of their combined scores. D Correlation of the REL with FN1 and ITGB3 mRNA expression in primary tumor samples from the TCGA_PAAD cohort. The graphs are adapted with data from the UCSC-Xena TCGA PAAD dataset. The Pearson r correlations are displayed in the figure for each target. E Patient TMA H-score correlation plots for c-Rel versus ITGB3 or FN1. F) Kaplan–Meier survival curves for patients in the TCGA dataset, divided by the median expression level, on the basis of either FN1 or ITGB3 expression. OS: overall survival, n= 178; DSS: disease-specific survival, n=172; DFI: disease-free interval, n=69; PFI: progression-free interval, n=178. The graphs are adapted with data from the UCSC-Xena TCGA PAAD dataset. G Patient TMA overall survival curves based on ITGB3 and FN1 H-scores. Statistical analysis is performed with the log-rank (Mantel‒Cox) test. Unless otherwise indicated, for all analyses, two-way ANOVA is used. Šídák's multiple comparison tests between genotypes are shown in the figures

    Article Snippet: pAY15 (OsTIR1(F74G) mAID-EGFP-NLS) was a gift from Masato Kanemaki (Addgene plasmid # 140534; http://n2t.net/addgene:140534 ; RRID: Addgene_140534) [ ].

    Techniques: Binding Assay, Fluorescence, Microscopy, Imaging, Stable Transfection, Transfection, Expressing, Cell Culture, Comparison